万寿菊油树脂制取高纯度叶黄素酯的工艺研究

Preparation of High-purity Lutein Esters from Marigold Oil

研究万寿菊油树脂中叶黄素酯的分离纯化过程。结果表明,较优的分离纯化条件为:丙酮溶解万寿菊油树脂的条件为:料液比1:3(m/V)、温度50℃、冷却时间3h;正丁醇处理丙酮浓缩物的条件为:料液比1:3.5(m/m)、温度50℃、冷却时间4h,正丁醇处理物再经乙醇洗涤,真空干燥,得纯度可达85%以上的叶黄素酯。

Acetone extraction followed by n-butanol purification was applied for preparing high-purity lutein esters from marigold oil. The extraction and the purification conditions were optimized using L9（33） orthogonal array design. The optimal extraction conditions were as follows： with a ratio of material to liquid of 1：3 （m/V） stirring at 50 ℃ for 30 min for the dissolution of marigold oil in acetone, and then cooling for 3 h, and the optimal conditions of n-butanol treatment for purification with a ratio of material to liquid of 1：3.5（m/m） stirring for the dissolution of crude lutein esters concentrate in n-butanol, and then cooling for 4 h. After ethanol washing and vacuum drying, lutein esters with over 85% purity were obtained.