利用超临界技术萃取石榴叶总酚工艺研究

Orthogonal Array Design as An Approach to Optimize Supercritical Fluid Extraction Conditions of Total Polyphenols from Punica granatum L. Leaves

利用超临界CO2装置对石榴叶中总酚提取工艺进行研究,以期得到高生理活性和高纯度的石榴叶活性物质。通过单因素和正交试验,得到最佳萃取条件为料液比1:2.5(g/ml)、萃取压力25MPa、萃取温度60℃、萃取时间120min。活性物质得率为0.1728%,纯度为42.61%。

Total polyphenols in Punica granatum L. leaves were extracted using supercritical CO2 fluid with the addition of absolute ethanol as a cosolvent. In order to maximize yield of total polyphenols, four crucial extraction parameters such as ratio of material to liquid, and extraction pressure, temperature and time were optimized by single-factor and orthogonal array methods. A maximum yield of total polyphenols at 42.61% purity was obtained by the 120 min extraction at 25 MPa and 60 ℃ with a ratio of material to liquid of 1：2.5.