微波法与超声破碎法提取蛹虫草菌丝体中虫草素的比较研究

Extraction of Cordycepin from Fermented Mycelia of Cordyceps militaris:A Comparitive Study of Ultrasonic-assisted and Microwave-assisted Procedures

分别就微波法和超声破碎法用于蛹虫草菌丝体中虫草素提取时应采取的工艺条件进行研究。采用单因素分析和正交设计试验分别研究提取溶剂、提取液pH值、微波和超声波的功率、提取时间对虫草素提取得率的影响。正交试验结果表明,微波提取法要好于超声波提取法。当以提取溶剂为水-乙醇(1:3,V/V)、pH7.0、微波功率500W,并提取3min的条件进行微波提取时,虫草素的提取得率最高。

Ultrasonic and microwave techniques were separately used to assist the extraction of cordycepin from fermented mycelia of Cordyceps militaris with ethanol. On the basis of single factor experiments, ultrasonic-assisted and microwave-assisted extraction procedures were optimized for maximizing yield of cordycepin using L9（34） orthogonal array design. The results showed that the assisting effect of microwave was better than that of ultrasonic, and a maximum yield of cordycepin was obtained under the following extraction conditions： microwave power, 500 W; extraction solvent, a mixture of water and absolute ethanol （1：3, WV） at pH 7.0; and extraction time 3 min.