摘要
以乙醇体积分数、浸提时间、固液比、提取温度为考察因素对玉米须总黄酮的提取条件进行正交设计试验,并用DPPH和FRAP两种方法测定了总黄酮的抗氧化活性。结果表明:玉米须总黄酮提取的最佳工艺条件为乙醇体积分数40%、提取时间2h、固液比1:25(m/V)、提取温度80℃,此时玉米须总黄酮的提取率为1.67%;玉米须总黄酮的抗氧化作用明显,对DPPH自由基的清除率达到77.18%,总抗氧化能力FRAP值为691.04μmol/L。
Total flavonoids in corn silk were extracted with ethanol and analyzed for their DPPH scavenging activity and total antioxidant capacity. The effects of ethanol concentration, extraction time, material/liquid ratio and extraction temperature were evaluated for optimizing the extraction procedure of total flavonoids. A maximum yield of total flavonoids of 1.67% was obtained by the 2 h extraction using 40% ethanol at 80 ℃. The total flavonoids from corn silk had marked antioxidant activities： the scavenging rate against DPPH free radicals was as high as 77.18% and the FRAP value reached 691.04 μ mol/L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第18期206-208,共3页
Food Science
关键词
玉米须
总黄酮
提取
抗氧化
corn silk
total flavonoids
extraction
antioxidant activities
