高效液相色谱法同时测定浓缩枣汁中环磷酸腺苷和环磷酸鸟苷的含量

Simultaneous HPLC Determination of cAMP and cGMP in Commercial Jujube Juice Concentrate

应用高效液相色谱法建立浓缩枣汁中环磷酸腺苷(cAMP)和环磷酸鸟苷(cGMP)的检测方法。结果表明,cAMP和cGMP在0～50mg/L线性范围内均具有良好的线性关系,方法检出限(LOD)为0.1mg/kg;两种环核苷酸的平均回收率在82.2%～99.3%之间,相对标准偏差(RSD)在2.1%～10.9%之间。本方法可有效地用于浓缩枣汁中cAMP和cGMP含量的测定。

A HPLC method was developed for the simultaneous determination of cAMP and cGMP in commerical jujube juice concentrate. Both concentrations of cAMP and cGMP exhibited a good linear relationship with peak area in the range of 0-50 mg/L. The limits of detection （LOD） of the method to them were both 0.1 mg/kg. The spiked recoveries at three levels （1, 10 and 20 mg/L） ranged from 82.2% to 99.3%, with a RSD between 2.1% and 10.9%. The method is suitable for the effective determination of cAMP and cGMP in jujube juice concentrate.