摘要
从湘潭南天化工厂的甲胺磷废水和污泥中分离细菌样品,以甲胺磷为唯一碳源和能源,经过定向筛选,得到1株可高效降解甲胺磷的菌株HN003,气相色谱测定其对甲胺磷的平均降解效率在24h、48h和72h分别达到45.8%、88.5%和100%。对其进行常规生理生化测试,结果表明,菌株HN003革兰氏染色为阳性,在10-42℃、pH3.0-pH14.0范围内均能生长,并与巴氏葡萄球菌的表型特征非常相似。为了进一步确定HN003的分类学地位,测定了其16S rRNA基因序列(1541bp),分析了相关细菌相应序列的同源性,构建了分子系统发育树,结果表明,菌株HN003与巴氏葡萄球菌的亲缘关系最近。综合上述结果,菌株HN003可鉴定为巴氏葡萄球菌(Staphylococcus pasteuri)。
Using methamidophos as the sole carbon and energy source, a bacteria strain HN003 was isolated and purified from methamidophos-polluted sludge from a local chemical factory discharge in Xiangtan, Hunan Province. The strain showed an efficient biodegrading ability to methamidophos. The methamidophos-degrading rate of this strain reached 45.8%, 88.5%, and 100% in 24 h, 48h, and 72h, respectively, determined in gas-chromatography. Results of traditional physiological and biochemical tests show that the strain is Gram-positive, and can grow in 10--42℃, and pH 3.0--14.0. The strain is very similar to Staphylococcus pasteuri in most of the phenotypes in traditional physiological and biochemical aspects, as well as in molecular phylogenetic dendrogram built from 1541bp 16S rRNA gene sequence with which they are compared. Therefore, strain HN003 was identified as S. pasteuri.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2009年第5期551-556,共6页
Oceanologia Et Limnologia Sinica
基金
国家"863"计划资助项目
2005AA219040号
湖南科技大学博士基金资助项目
E50437号
关键词
甲胺磷
巴氏葡萄球菌
降解
生理生化测试
16S
RRNA基因
Methamidophos, Staphylococcus pasteuri, Degradation, Physiological and biochemical tests, 16S rRNA gene