速尿对实验性膜迷路积水前庭碳酸酐酶活性的影响

Effect of furosemide on the carbonic anhydrase activity in vestibule

为进一步探讨速尿对膜迷路脱水的机理，采用组织、细胞化学染色法，观察速尿对壶腹嵴毛细胞、前庭移行细胞、暗细胞及椭圆囊毛细胞碳酸酐酶的影响。结果显示，碳酸酐酶主要分布于半月面移行细胞侧膜和前庭暗细胞指状突胞膜；实验性膜迷路积水耳碳酸酐酶分布与正常耳无显著变化；速尿对前庭暗细胞、移行细胞的碳酸酐酶有明显抑制作用。表明抑制碳酸酐酶是速尿对内淋巴脱水的作用机理之一。

In order to explore the mechanism that the furosehade dehydrates and improves the vestibular function in the endolpophatic hydrops. The effects of furosetale on the carbonic anhy-drase activity in vestibule were studied by using histocytochemistry and image analysis- The results demonstrated that the furosemide can obviously inhibit the carbonic anhydrase activity. There was no significant difference between the normal ear and the ear with endolymphatic hydrops in the lo-cation of the carbonic anhydrase in the vestibule. It suggested that one of the dehydrant mecha-nisms is inhibition of carbonic anhydrase activity.