胃癌组织FAT10与突变型p53表达及其相互关系

Expressions of FAT10 and p53 mutant in human gastric cancer and their relations

目的检测FAT10与突变型p53在胃癌组织中的表达情况及相互间关系。方法采用免疫组化和逆转录-聚合酶链反应（RT—PCR）方法检测FAT10和突变型p53在62例胃癌患者的胃癌组织、癌旁组织（距癌边缘2～5cm）及正常胃组织（距癌边缘〉5cm）中的表达水平，统计分析两者表达间的关联性及FAT10与胃癌预后之间的关系。结果FATl0蛋白和突变型p53蛋白在胃癌组织中的表达阳性率分别为51．61％（32／62）和45．16％（28／62），显著高于癌旁组织[12．90％（8／62）和14．51％（9／62），χ^2值分别=21．26和20．69，P值均〈0．01]及正常胃组织[6．45％（4／62）和9．68％（6／62），χ^2值分别=13．91和19．61，P值均〈0．01]。胃癌FAT10蛋白及mRNA的表达上升主要与胃癌的淋巴转移及TNM分期密切相关（P值均〈0．05）。胃癌组织中FAT10表达在蛋白及基因水平均与突变型p53表达呈正相关性（r值分别=0．865和0．761，P值分别〈0．05和0．01）。FAT10蛋白阳性表达或mRNA表达水平高者，其累计生存时间显著低于FAT10阴性者（P值均〈0．05）。结论FAT10与突变型p53在胃癌组织中表达上调且呈正相关性，两者可能共同参与胃癌的形成与发展，FAT10对评价胃癌的预后可能有较好的价值。

Objective To investigate the expressions of FAT10 and p53 mutant in gastric cancer tissues and their relations. Methods Immunohistochemistry and RT-PCR were used to detect the expressions of FAT10 and p53 in gastric cancer tissues （n= 62）, para-cancerous tissues （2- 5 cm apart from cancer, n=62）, and normal gastric tissues （〉5 cm apart from cancer, n =62）. The association of FAT10 with p53 and clinical outcomes were analyzed by Spearman and Pearson correlation. Results The immunohistochemistry examination showed that expressions of FAT10 [51.61%（32/62）] and p53 [45.16%（28/62）] were significantly higher in cancerous tissues than in paracancerous tissues [12.90%（8/62） and 14. 51%（9/62）, χ^2=21. 26 and 20.69, P〈0.01] and normal tissues [6.45% （4/62） and 9.68% （6/62）, χ^2= 13.91 and 19.61, P 〈 0.01]. Overexpressions of FAT10 protein and mRNA in cancerous tissues were closely related to lymph node metastasis and TNM staging （both P value 〈0.05）. There was a positive correlation between FAT10 and p53 in protein andmRNA expressions （protein r =0.865, P 〈0.05; mRNA r=0.761, P〈 0.01）. Those with positive expression of FAT10 had lower survival rate compared to those with negative expression （P 〈 0. 05）. Conclusions The positive relation between over expression of FAT10 and p53 implicates that both are involved in the gastric carcinogenesis, and FAT10 is a novel gastric cancer marker with prognostic significance.