亚慢性锰中毒对公鸡肝脏的毒性作用

Effect of Subchronic Manganism on Liver in Cocks

为探讨染锰对公鸡肝脏的毒性作用,将50日龄海兰褐公鸡400只,随机分为4组,分别在饲料中添加0、600、900、1800mg/kgMnCl2建立亚慢性锰中毒模型,于30、60、90d采取肝脏,观察肝脏的显微及超微结构,检测肝脏中抗氧化酶(GSH-Px、SOD)活性,MDA含量。结果90d染锰公鸡肝组织的显微结构超微结构均表现出不同程度的病理学变化,各时间点肝脏GSH-Px、SOD活性基本呈降低趋势,MDA含量逐渐升高,均具有统计学意义(P<0.01,P<0.05)。亚慢性锰中毒使肝细胞发生了病理学改变并且降低了肝脏的抗氧化能力。

The aim of this study was to investigate the effect of manganese on the liver in cocks. 400 fifty-day-age Hy-Line brown cocks were randomly divided into four groups. The 0,600,900,1 800 mg/kg MnCl2 were respectively added into the basic diet to establish the subchronic manganism model. After 30,60 and 90 days treatment,the livers in every group were collected. The microstructure and ultrastructure of livers were observed. The antioxidase （GSH-Px,SOD） activities and the MDA content in livers were detected. The livers in the 90-day treatment group took on different degrees of pathological changes by the observation of microstructure and uhrastructure. Additionally,at every time point, the GSH-Px and SOD activities in the liver took on a decreased tendency,and the MDA content increased gradually （P〈0.01,P〈0.05）. The subchronic manganism could cause the pathological changes and decrease antioxidation of the liver in cocks.