磺酸基邻苯二甲酰亚氨甲基酞菁锌C对肿瘤的光动力学效应研究

Photodynamic therapy of sulfonated phthalimidomethyl Zinc phthalocyanine C on tumors

目的:研究新型光敏剂磺酸基邻苯二甲酰亚氨甲基酞菁锌C(ZnPcSP-C)对肿瘤的光动力效应(PDT).方法:细胞经药物作用2h,用红光照射后继续培养24h,以台盼蓝排染法测其PDT杀伤作用;荷瘤鼠给药后以红光照射瘤块,1周后测其抑制瘤率.结果:ZnPcSP-C对K_(562)、Be17402 PDT的 CI_(50)分别为1.7,2.8μg/ml;6mg/kg ip对U_(14)荷瘤鼠的抑瘤率为67.2%.结论:ZnPcSP-C对肿瘤有较强的体内外光动力效应.

OBJECTIVE:To study the effect of photodynamic therapy(PDT) of sulfonated phthalimidomethyl zinc phthalocyanine C(ZnPcSP-C) on tumors.METHODS: After tumor cells exposed to the drug for 2h were irradiated with red light, then cultured for 24h,the PDT effect of the drug was determined by trypan blue dye exclusion. After administration of the drug, the mice bearing tumor was irradiated with red light, then the inhibitory rate of tumor was determined. RESULTS:IC50 of the drugs with PDT on K562 and Bel 7402 cell lines were 1.7, 2.8μg/ml respectively. U14 cervical carcinoma in mice was inhibited by 67. 2% after ip the drug 6mgAg with PDT. CONCLUSION: ZnPcSP-C has a potential effect of PDT on tumors in vitro and vivo.