反相高效液相色谱法测定小儿清热灌肠剂中6种成分的含量研究

Determination of six constituents in Xiaoerqinre sausage by RP-HPLC

目的:建立了测定小儿清热灌肠剂中6种成分含量的方法.方法:反相高效液相色谱法.以ODS(5μm,4.6mm×250mm)为色谱柱,流动相A:CH_3OH-H_2O-HCOOH(10:90:1),流动相B:CH_3OH-H_2O-HCOOH(80:20:1),检测波长为280nm.结果:柴胡皂甙A含量为0.4190,加样回收率为90.1% ±2.2%,n=6,RSD=2.4%,番泻甙A含量为0.7790,加样回收率为93.99%±3.8%,n=6,RSD=4.0%,苦参碱含量为0.1290,加样回收率为100.6%±2.7%,n=6,RSD=2.9%,大黄素甲醚含量为0.3390,加样回收率为95.8%±1.4%,n=6,RSD=1.5%,大黄酸含量为0.2590,加样回收率为91.3%±2.1%,n=6,RSD=2.3%,大黄素含量为0.3190,加样回收率为94.6%±3.3%,n=6,RSD=3.5%.结论:该方法简便,重现性好.

OBJECTIVE: A reversed - phase HPLC method was established for determination of six constituents in Xiaoer.METHODS:A HPLC method was developed under the following conditions:flow rate:1.0mo/min,the UV detector was set at 280nm, the mobile phase A was methanol - water - formic acid (10:901), the mobile phase B was methanol-water - formic acid(80:20:1) .RESULTS:The quantity of skaiko - side A was 0.4190 the recovery was 90.1% ± 2.2%, n = 6,the relative standard deviation(RSD) was 2.4% ,the quantity of sennoside A was 0.7790,the recovery was 93.99% ±3.8%(n = 6),the RSD was 4.0%,the quantity of matrine was 0.1290,the recovery was 100.6%±2.7%(n = 6),the RSD was 2.9%,the quantity of physcion was 0.3390,the recovery was 95.8%±1.4%(n = 6),the RSD was 1.5%,the quantity of rhein was 0.2590,the recovery was 91.3% ±2. 1%(n = 6),the RSD was 2.3%,the quantity of emodin was 0. 3190,the recovery was 94.6%±3.3%(n = 6), the RSD was 3. 5%. CONCLUSION: This method was simple and showing good reproducibility.