大叶蒟叶的化学成分研究

Chemical constituents in leaves of Piper laetispicum

目的研究大叶蒟Piper laetispicum叶子的化学成分。方法采用硅胶柱色谱和大孔吸附树脂分离纯化,通过波谱方法鉴定化合物结构。结果从大叶蒟的叶中分离得到16个化合物,分别鉴定为(2E,4E)-N-isobu-tyl-11-phenylundecadienamide(Ⅰ)、(2E,4E)-N-isobutyl-15-phenylpentadecadienamide(Ⅱ)、大叶蒟素(lae-tispicine,Ⅲ)、(2E,4E)-N-isobutyl-7-(3′,4′-methylene-dioxyphenyl)heptadienamide(Ⅳ)、短穗胡椒酰胺A(brach-ystamide A,Ⅴ)、dihydropipercide(Ⅵ)、粗梗胡椒酰胺A[(pipermacramide A,2E,4E)-N-isobutyleicosadienamide,Ⅶ]、(+)-匙叶桉油烯醇[(+)-spathulenol,Ⅷ]、d-芝麻素(d-sesamin,Ⅸ)、胡椒醇(piperitol,Ⅹ)、牡荆苷(vitexin,Ⅺ)、vitexin-2″-O-β-D-glucoside(ⅩⅡ)、N-p-coumaroyltyramine(ⅩⅢ)、β-谷甾醇(β-sitosterol,ⅩⅣ)、豆甾醇(stigmas-terol,ⅩⅤ)、正三十二醇(1-dotriacontanol,ⅩⅥ)。结论化合物Ⅰ、Ⅱ为首次获得的天然产物,化合物Ⅴ、Ⅵ、Ⅷ为首次从大叶蒟中分离得到,化合物Ⅺ、ⅩⅡ和ⅩⅥ为首次从胡椒属中分离得到。

Objective To study the chemical constituents in the leaves of Piper laetispicurn. Methods Compounds were separated and purified by silica gel column and macroporous resin. Their structures were identified on the basis of spectral methods. Results Sixteen compounds were isolated and identified from the leaves of P. laetispicum： （2E, 4E）-N-isobutyl-11-phenylundecadienamide （ Ⅰ ）, （2E, 4E）-N- isobutyl-15-phenylpentadecadienamide （ Ⅱ ）, laetispicine （ Ⅲ ）, （2E, 4E）-N-isobutyl-7-（3′, 4′-methylene- dioxyphenyl） heptadienamide （ Ⅳ ）, brachystamide A （ Ⅴ ）, dihydropipercide （ Ⅵ ）, pipermacramide A [- （2E, 4E）-N-isohutyleicosadienamide, Ⅶ ], （＋）-spathulenol （ Ⅷ ）, d-sesamin （ Ⅸ ）, piperitol （ Ⅹ ）, vi- texin （Ⅺ）, vitexin-2″-O-13-D-glucoside （ Ⅻ ）, N-p-coumaroyhyramine （ ⅩⅢ ）, β-sitosterol （ ⅩⅣ）, stigmasterol （ⅩⅤ）, and 1-dotriacontanol （ⅩⅥ）. Conclusion Compounds Ⅰ and Ⅱ are isolated for the first time as natural substance, compounds Ⅴ , Ⅵ, and Ⅷ are first isolated from P. laetispicurn, compounds Ⅺ, Ⅻ , and ⅩⅥ are obtained from the plants of Piper L. for the first time.