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RP-HPLC法测定转基因贯叶连翘植株中褪黑素 被引量:2

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摘要 目的建立测定褪黑素的反相高效液相色谱紫外分析方法,分析并测定通过组培获得的贯叶连翘不同转基因株系及其亲本植株的褪黑素。方法以SymmetryC18(150mm×4.6mm,5.0μm)为色谱柱;100mmol乙酸铵-甲醇(80∶20)为流动相,体积流量为0.8mL/min;检测波长为265.8nm,灵敏度为2.00AUFS。结果褪黑素浓度在20~500ng/mL线性关系良好(R2=0.9992),平均加样回收率为98.09%(n=6),RSD为2.21%,最小检出量为1.0ng/mL。褪黑素峰保留时间约为8.8min。结论该方法可以简便准确分析不同株系贯叶连翘中的褪黑激素的量;贯叶连翘YXu55(含庆大霉素抗性标记和AANAT-HIOMT基因)转基因株系的褪黑素的量均高于pZP122(仅含庆大霉素抗性标记,不含AANAT-HIOMT基因的空白质粒)转基因株系和未转基因的对照植株;而pZP122转基因株系褪黑素的量跟未转基因的对照植株的量几乎相等。
出处 《中草药》 CAS CSCD 北大核心 2009年第10期1651-1653,共3页 Chinese Traditional and Herbal Drugs
基金 国家自然科学基金资助项目(30671082) 陕西省自然科学基金资助项目(2007C104) 中国科学院西部之光基金资助项目(2008DF01) 陕西省教育厅基金资助项目(09JK775)
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参考文献8

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