微孔膜乳化法制备大粒径琼脂糖微球

Preparation of Large Size Agarose Beads by Microporous Membrane Emulsification Technique

以6%的琼脂糖溶液为水相,不同体积配比的液体石蜡(LP)和石油醚(PE)的混合溶液为油相,PO-5S为乳化剂,采用微孔膜乳化法制备了平均粒径为90μm的琼脂糖微球.考察了SPG膜孔大小、油相组成、反应温度、压力等因素对成球粒径及其分布的影响.结果表明,在使用膜孔为25.9μm的微孔膜、LP/PE体积比为11:1及65℃的条件下可制得均一的大粒径琼脂糖微球,微球平均粒径为93.3μm,粒度分布系数为1.25,且各批产品的相对标准偏差仅为1.34%,产品重复性良好.

With 6% agarose solution as water phase, composed by liquid paraffin （LP） and petroleum ether （PE） with different volumetric ratios as oil phase, and PO-5S as emulsifier, agarose beads with average size of 90 μm were prepared by membrane emulsification, and the effects of pore size of the shirasu porous glass （SPG） membranes, oil phase composition, temperature and pressure on the size and size distribution of prepared agarose beads were investigated. The optimal preparation conditions were： the pore size of used SPG membrane was 25.9μm, oil phase composed by volumetric ratio of LP and PE 11：1 and the preparation temperature 65 ℃. The prepared agarose beads with good reproducibility had an average diameter of 93.3 μm, size distribution index, Rspan, 1.25 and relative standard deviation 1.34%.