光合细菌与产气肠杆菌协同产氢特性分析

Characteristic Analysis of Cooperation Hydrogen Production Using Rhodopseudomonas sp. DT and Enterobacter Aerogenes

对光合细菌(Rhodopseudomonas sp.DT)与产气肠杆菌(Enterobacter aerogenes)进行了发酵产氢试验,考察了不同起始接种比例、培养温度及碳源条件下混合菌协同产氢特性。结果表明:光合细菌与产气肠杆菌初始接种比例对协同产氢影响较大,初始接种比例为1:1最有利于协同产氢,产氢效率和产氢周期达到了3.1molH2/mol葡萄糖及81h。进一步培养液pH动力学变化研究发现初始接种比例为1:1的混合菌培养液pH变化较小,为pH6~7,利于混合菌协同产氢。28°C^37°C温度范围混合菌可保持较高的协同产氢活性,20°C条件下混合菌协同产氢效率低,50°C条件下不能产氢。混合菌可利用葡萄糖、琥珀酸、苹果酸以及可溶性淀粉为碳源产氢。葡萄糖为最佳产氢碳源,底物转化率为36.11%。利用可溶性淀粉产氢,转化率仅为8.22%,该结果表明协同作用可拓展对多种产氢碳源的利用。

Cooperation hydrogen production was carried out using Rhodopseudomonas sp. DT and Enterobacter aerogenes. The effects of the initial ratio of Rhodopseudomonas sp. DT and E. aerogenes, culture temperature, and carbon source on the cooperation hydrogen production were investigated. The results suggested that cooperation hydrogen production rate was highly affected by the initial ratio of Rhodopseudomonas sp. DT and E. aerogenes. The mixed bacteria of Rhodopseudomonas sp. DT and E. aerogenes with 1：1 initial ratio benefited to the cooperation hydrogen production, which led the hydrogen production rate and duration of gas production to 3.1 mot H2/mol glucose and 81 h, respectively. The pH dynamics analysis of culture medium further discovered that the pH of the mixed bacteria with 1：1 initial ratio changed from 6 to 7 smaller than other conditions, which was probably fitted to produce hydrogen. Furthermore, the mixed bacteria with 1：1 initial ratio had the higher hydrogen production efficiency at temperatures of 28℃ and 37℃ than at 20℃, and without any hydrogen production at temperature of 50℃. The carbon sources of glucose, succinate acid, malic acid could be used to produce hydrogen by the mixed bacteria. Even the solu-ble starch, unused by Rhodopseudomonas sp. DT, was also decomposed by the mixed bacteria to produce hydrogen with the conversion efficiency of 8.22%. The glucose was the optimal carbon resource, and the conversion efficiency could reach to 36.11%. The results, further, implied that the cooperation hydrogen production could enlarge the use of the carbon sources.