HPLC法测定保健食品中人参皂苷Rh_2的含量

Determination of ginsenoside Rh_2 in health food by HPLC

目的：探讨高效液相色谱（HPLC）法测定保健食品中人参皂苷Rh2的含量。方法：采用C18色谱分析柱;流动相为乙腈：水=60：40;紫外检测器（或二极管阵列检测器）,检测波长203 nm。结果：在0.05-5μg范围内,Rh2线性关系良好,相关系数r=0.99999,平均回收率为99.3%,平均标准偏差（RSD）为1.86%,最低检出限为1.8 ng。结论：本方法简便、快速、可靠,能满足保健食品中Rh2含量测定的需要。

Objective：To develop a method to determinate of ginsenoside Rh2 in health food by HPLC. MethodS：C18 column was used to determine the ginsenoside Rh2. The mobile phase was： acetonitrile -water（ 60：40 ）. The ginsenoside Rh2 was detected by UV（ or diode array detection） at 203 nm. Results： The linear range was 0. 05 - 5 μg with correlation coefficient of 0. 99999, the average recovery of the method was 99. 3% , the relative standard deviation（RSD） was 1.86% and its monitoring limit was 1.8 ng. Conclusion：This method is convenient, fast and accurate and it can meet the analysis of ginsenoside Rh2 in health food.