去甲氯胺酮胶体金免疫层析检测技术的研究

Development of an immunochromatographic assay for detection of norketamine

目的:建立一种准确、快速、简便的检测去甲氯胺酮(NKET)的胶体金免疫层析检测技术。方法:采用柠檬酸三钠还原法制备一定粒径的胶体金溶液,并标记抗NKET单克隆抗体,将一定浓度的包被抗原(NKET-OVA)及羊抗鼠二抗IgG喷在硝酸纤维素膜上,制备胶体金免疫层析试纸条。结果:该试纸条检测阈值可达500 ng/ml,检测的准确性为97.2%,试纸条的稳定性和重复性良好。结论:采用该试纸条检测去甲氯胺酮特异性强,灵敏度高,而且简便快速,无需特殊仪器设备,在毒品滥用筛查工作中应用价值较大。

Objective：To develop an accurate, rapid and simple colloidal gold immunochromatagraph assay （GICA） for the detection of norketamine （NKET）. Methods：Colloidal gold was prepared through reducing HAuC14 · 3H2O by sodium citrate and conjugated with anti - norketamine monoclonal antibody. The optimal concentration of NKET - OVA and sheep anti - mouse IgG were coated on nitrocellulose membrane to get the test strip. Results：The cut - off value was 500 ng/ml and the accuracy was 97. 2%. The stabilization and repetition performed well. Conclusion ： The colloid gold strip for detecting norketamine is a specific, sensitive and rapid assay for the simultaneous and qualitative detection of drug abuse.