石墨炉原子吸收法测定血清中的铬

Determination of chromium in serum with graphite furnace atomic absorption spectrometry

目的：建立人血清中痕量铬测定的新方法。方法：在血清样品中加入一定量的硝酸溶液使其酸度为0.5%,以硝酸镁为基体改进剂,石墨炉原子吸收光谱法测定血清中的铬含量。结果：本方法检出限为0.07μg/L,相对标准偏差为2.23%,本方法实测回收率为92%-105%。结论：该方法操作简单,测定迅速,无需复杂的样品前处理,精密度和准确度较好,值得广泛推广。

Objective： To establish a new method of detecting and studying the trace chromium in serum. Methods： Take 0.5% of nitric acid to nitrify the serum, and use magnesium nitrate as matrix modifiers to eliminate the interference, and with Graphite furnace atomic absorption to detect. Results： The detection limit of chromium was 0. 07 μg/L, the relative standard deviation was 2. 23%. The racovery rate was 92% - 105%. Conclusion：This method is simple and fast and with high sensitivity and nicety, dispensing with complicated anterior steps.