466份布鲁氏菌病血清学凝集试验结果分析

Analysis of serum agglutination test for 466 serum samples of Brucellosis

目的:了解虎红平板凝集试验(RBPT)和试管凝集试验(SAT)在布病抗体检测中的应用。方法:采用虎红平板凝集试验(RBPT)和试管凝集试验(SAT)两种方法对466份临床上有典型病症患者及与羊、牛、猪有密切接触的饲养员和屠宰工人的血清进行检测。结果:9份RBPT和SAT均为阳性;12份RBPT阳性,SAT阴性;2份RBPT阴性,SAT阳性;443份RBPT和SAT均为阴性。结论:虎红平板凝集试验(RBPT)主要检查IgG类抗体,而试管凝集试验(SAT)主要以检测IgM类抗体为主。在应用两种方法进行布病诊断时必须结合流行病学接触史和临床症状体征认真分析谨慎判断。

Objective：To study the application of RBFF and SAT in the detection of Brucellosis. Methods： RBFF and SAT were used to detect the serum samples from 466 typical patients and the feeders who contacted with sheep, cow or pig intimately. Results：The RBFF and SAT of 9 serums were both positive in total 466 serums. 12 serums were positive and negative in RBPT and SAT respectively; 2 serums were negative and positive in RBPT and SAT respectively. The RBPT and SAT of the rest 443 serums were both negative. Conclusions ： RBPT is used in detection of IgG, while SAT is used in detection of IgM. To diagnose the RBPT and SAT of Brucellosis must be considered with the epidemiological contact history and clinical symptom.