摘要
利用琥珀酸酐法对DON分子进行结构修饰,采用高效液相色谱法和薄层层析法进行检测,并将衍生物与载体蛋白牛血清白蛋白进行偶联,设计人工抗原,通过紫外分光光度法、SDS-PAGE和ELISA试剂盒分析偶联结果。结果显示,成功得到了DON-BSA,偶联物中DON浓度为0.797μg/μL。
Deoxynivalenol （DON） molecular structure was modified with succinic anhydride （SA） method in this study. HPLC and TLC were used to determine the product. The derivative and carrier protein BSA were coupled to develop artificial antigen. The conjugate was ana- lyzed with ultraviolet spectrophotometry, SDS-PAGE and ELISA kit for DON. The result showed that DON artificial antigen was obtained and the content of DON in DON-BSA was 0. 797μg/μL.
出处
《畜牧与兽医》
北大核心
2009年第10期1-4,共4页
Animal Husbandry & Veterinary Medicine
基金
科技部"863"项目(2007AA10Z429)
关键词
脱氧雪腐镰刀菌烯醇
人工抗原
鉴定
deoxynivalenol （DON）
artificial antigen
identification
