摘要
采用混合酸酐法将短裸甲藻毒素(brevetoxins,BTX)分别与卵清蛋白(OVA)和牛血清蛋白(BSA)偶联制备人工抗原。以BTX-BSA作为免疫抗原免疫BALB/c小鼠,通过细胞融合-ELISA筛选抗短裸甲藻毒素的单克隆抗体,并对其特异性和灵敏性进行了鉴定。结果获得了1株可分泌短裸甲藻毒素单克隆抗体的杂交瘤细胞株3B4,该株杂交瘤细胞腹水抗体效价达10-5,与其他类似物没有交叉反应。该抗体为建立短裸甲藻毒素免疫学检测方法奠定了基础。
Brevetoxin （BTX） was conjugated with bovine serum albumin （BSA） and ovalbumin （OVA） by mixed anhydride coupling method. BTX-BSA and BTX-OVA were used as immunizing antigen and coating antigen, respectively. BTX-BSA was vaccinated into Balb/ c mice for monoclonal antibody （McAb） production. A hybridoma （named 3B4） which secrected McAb against brevetoxin was obtained and some characteristics of the McAb were studied. The antibody titer was 10^-5. And the McAb has no cross reactions with other marine toxins. The McAb lays a foundation for the establishment of an immunological detection method on brevetoxin.
出处
《畜牧与兽医》
北大核心
2009年第10期5-7,共3页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金资助项目(30771657)
关键词
短裸甲藻毒素
单克隆抗体
鉴定
brevetoxin
monoclonal antibody
identification
