檵木中槲皮素、山奈酚和杨梅素含量的高效液相色谱法测定

Simultaneous Determination of Quercetin,Kaempferol and Myricetin in Loropetalum chinense(R. Brown) Oliv. by High Performance Liquid Chromatography

建立了同时测定檵木中槲皮素、山奈酚和杨梅素含量的反相高效液相色谱测定方法,色谱柱为UltimateTMXB-C18(150mm×4.6mm,5μm),流动相是体积比为32∶68的乙腈/0.4%磷酸,淋洗速度1.0mL/min,检测波长360nm,对3种样品的线性工作范围均为0.072～2.16(μg)(R2=0.9999),平均加样回收率(n=6)分别为99.1%、97.0%和97.9%,回收率测定标准偏差分别为1.45%、1.38%和2.04%。所建方法稳定可靠,可用于檵木的质量控制。

A method based on reversed phase high performance liquid chromatography was developed to simultaneously determine the content of Quercetin,Kaempferol and Myricetin in the Loropetalum chinense（R. Brown）Oliv. Ultimate ^TM XB-C18（150mm×4.6mm,5μm） column was used and eluted at 25 ℃ with a mobile phase of acetonitrile and 0.4% phosphoric acid （32∶68,v/v） at a flow rate of 1. 0 mL /min. The calibration curves were constructed between peak area measured at 360 nm and the injected quantity,giving a linear working rang of 0.072 - 2.16 （μg） （ R ^2 = 0.9999） for all the three analytes. The spiked recoveries （n = 6） were 99.1%, 97.0% and 97.9% with RSD of 1.45%, 1.38% and 2.04%, respectively. This method is considered to be stable, accurate, reproducible and suitable for quality control of Loropetalum chinense （ R . Brown ） Oliv.