釉鞘蛋白在小鼠牙胚发育中的时空表达

Spatio-temporal Expression of Ameloblastin During Odonto genesis in Mus musculus

目的:观察釉鞘蛋白(ameloblastin)在小鼠牙胚发育中表达的时空顺序及分布,探讨釉鞘蛋白在釉质发育矿化中的作用。方法:采用原位杂交的实验方法,观察釉鞘蛋白在牙胚发育的增殖分化期、分泌初期及分泌期的表达。结果:釉鞘蛋白mRNA在成釉细胞增殖分化期表达为阴性,分泌初期细胞内及新生釉基质中开始出现弱阳性表达;至分泌期,细胞内及新生釉基质中均呈强阳性表达。成熟釉质中无釉鞘蛋白mRNA的阳性表达。结论:本实验从分子水平上进一步明确了釉鞘蛋白的时空表达,并推测釉鞘蛋白可能介导了釉质的发育矿化,它与牙齿发育、釉基质矿化反应等过程密切相关。

Objective：To study the expression of ameloblastin in different development stages of Mus musculus tooth germ aud to disscuse the function of ameloblastin in the development of enamel. Methods：We performed in situ hybridization after preparing specimens of different stages of the incisor developing tooth germ in Mus musculus. Results：Ameloblastin mRNA was negative under microscope in the ameloblast proliferate differentiation period, it showed weakly positive expression in intra-cellular and new-birth enamel matrix in secrete initial stage while it showed strongly positive expression in intra-cellular and new-birth enamel matrix when it came to secretion phase. There was no positive expression of ameloblastin mRNA in mature stage. Conclusion ： In this study, we further clarified the spatio-temporal expression of ameloblastin from the molecular level. The results suggest that ameloblastin may mediate the development of enamel matrices and mineralization process.