摘要
目的探讨邻苯二甲酸二丁酯(DBP)对大鼠肝脏细胞的急性氧化损伤作用。方法用0、5、20、80μmo·lL-1的DBP溶液对大鼠肝脏细胞进行体外染毒1h,然后分别用硫代巴比妥酸(TBA)法和KCl-SDS沉淀法来检测丙二醛(MDA)含量和DNA-蛋白质交联(DPC)系数的变化。结果随着DBP染毒浓度的升高,大鼠肝脏细胞的MDA含量和DPC系数逐渐上升,且在5μmo·lL-1浓度时就与对照组具有良好的统计学意义(P<0.01)。结论DBP能够对体外大鼠肝脏细胞造成氧化损伤。
Objective The present study was conducted to study the effects of Di-n-butyl phthalate （DBP） on the acute oxidative damage in liver cells of rat. Methods Liver cells of rat were exposed to different concentration of DBP （0, 5,20 and 80 μmo·lL^-1） for one hour in vitro. Thiobarbituric acid （TBA） assay and KCl-SDS assay were used to measure the change of the contents of malondialdehyde （MDA） and the coefficient of DNA-protein crosslinks （DPC） respectively. Results With statistical significance, both MDA contents and DPC coefficient in liver cells increased gradually with the increase of DBP concentrations. Conclusion These results suggested that DBP could induce the acute oxidative damage in liver cells of rat.
出处
《公共卫生与预防医学》
2009年第4期4-7,共4页
Journal of Public Health and Preventive Medicine
基金
国家科技支撑计划项目(No.2006BAJ02A10
No.2006BAI19B05)
关键词
邻苯二甲酸二丁酯
肝脏细胞
氧化损伤
丙二醛
DNA-蛋白质交联
Di-n-butyl phthalate （DBP）
Liver cells
Oxidative damage
Malondialdehyde （MDA）
DNA-protein crosslinks （DPC）
