心复康促进血管新生对移植骨髓间充质干细胞的保护作用

XiFuKang to Promote Angiogenesis in the Transplanted Bone Marrow-Derived Mesenchymal Stem Cells, the Protective Effect

目的:探讨中药心复康在大鼠急性心肌梗死后对移植骨髓间充质干细胞(BMSCs)的保护作用及其机制。方法:经密度梯度离心联合贴壁筛选法培养并纯化BMSCs,采用冠状动脉结扎法建立大鼠急性心肌梗死模型,移植前以5-溴脱氧尿核苷(Brdu)对移植细胞进行标记,术后采用中药心复康灌胃联合干细胞移植治疗。移植后2周ELISA法检测大鼠血清血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)含量;处死动物,免疫组织化学方法检测心肌损伤区VEGF、bFGF蛋白表达情况、Brdu阳性移植细胞数和CD34+新生血管数。结果:心复康联合干细胞移植治疗大鼠心肌梗死,能提高移植细胞存活率(与细胞移植组比较P<0.05),提高大鼠血清VEGF、bFGF含量(与细胞移植组比较P<0.05),提高梗死区VEGF、bFGF蛋白表达,增加梗死区新生血管数量。结论:中药心复康对大鼠急性心肌梗死后移植干细胞有明显的保护作用,其机制可能与心复康联合干细胞移植能更有效的促进损伤区血管新生有关。

Objective： To discuss the protective action on transplanted BMSCs by Chinese traditional medicine XinFuKang（XFK） after rats acute myocardial infarction.Methods： BMSCs were cultured and purified by gradient centrifugation -adhesive method. The rats with acute myocardial infarction were builted by coronary artery ligation. Before transplation, BMSCs were signed by Brdu, and after the OPS of coronary artery ligation, the therapy methode was XFK combining BMSCs transplantation. After 2 weeks, we measured serum VEGF and bFGF by method of ELISA, the albumen VEGF and bFGF, cell amount of Brdu（＋） and neonatal vessels of CD34^＋ around the infarct area by method of IHC. Results： The method of XFK combining BMSCs transplantation can increase livability of transplanted BMSCs and serum VEGF and bFGF （compared with group of BMSCs tranplation ,P〈0.05）, enhance infarct area albumen VEGF. bFGF and neonatal vessels. Conclusion： Compared with simply BMSCs transplantation, XFK have better protection on the transplanted BMSCs, the mechanism maybe XFK can promote the vessels regeneration of infarct area.