小鼠背部慢性皮炎—湿疹模型的初步研究

Primary Study on Model of Chronic Dermatitis-eczema in Mice on its Dorsum

目的:初步建立小鼠背部慢性皮炎-湿疹模型。方法:用二硝基氯苯同时建立小鼠背部及耳廓慢性皮炎-湿疹模型,比较两种模型的皮肤肿胀度、表面皮损及组织病理变化。同时参照文献建立急性接触性皮炎模型进行比照。结果:耳部及背部模型组皮肤表面均呈现片状红斑、角化、表面结痂,皮损情况及病理组织像与对照组、急性模型组均有显著差别。结论:低浓度DNCB反复刺激小鼠背部皮肤可建立慢性皮炎-湿疹模型。

Objective：Preliminary establish the model of chronic dermatitis-eczema in mice on its dorsum. Methods：To build up dermatitis-eczema molel in mice both on its auricle and dorsum,then compare its dermal swelling, dermal damage and histopathology.At the same time,the model of acute allergic contact dermatitis（ACD）is build up to be compared with.Results：It was showed flake erythema, keratinization, incrustation on the derma of both the auricle-model group and the dorsum-model group . There were remarkable differences on there dermal damage and histopathology in different groups. Conclusion：Model of chronic dermatitis-eczema in mice on its dorsum could be built by repetitive stimimulation of low concentration DNCB.