摘要
目的研究P13K和基质金属蛋白酶-7(MMP-7)在食管鳞癌组织(ESCC)中的表达及其与食管鳞癌细胞发生、发展与转移的关系以及两者之间的相关性。方法应用免疫组织化学sP法检测24例正常食管黏膜、94例食管鳞癌组织中P13K和MMP-7的表达情况。结果食管癌组织中P13K和MMP-7的阳性表达率分别为71.28%(67/94)和52.13%(49/94),与正常食管黏膜组织阳性表达率4.17%(1/24)和0%(0/24)相比,差异均有统计学意义(P均〈0.01);P13K蛋白的表达与食管癌细胞的分化程度、淋巴结转移、浸润深度及临床分期显著相关(P均〈0.01);MMP-7表达率与淋巴结转移(P〈0.05)、分化程度(P〈O.05)、浸润深度(P〈0.01)和I临床分期(P〈0.05)等因素有关;P13K与MMP-7表达呈显著正相关(r=0.232,P=0.025)。结论P13K、MMP-7表达与食管癌发生、发展及侵袭转移有关。
Objective To study the expression of PI3K and MMP-7 in esophageal carcinoma and the relationship between the expression of PI3K and MMP-7 and carcinogenesis and progression of esophageal carcinoma. Methods PI3K and MMP-7 expression were detected in 24 normal esophageal mucosa,94 primary tumor tissues with SP immunohistochemistal method. Results There were significant differences of PI3K and MMP-7 expressions between esophageal carcinoma and normal mucosa epithelium ( all P 〈 0. 01 ) [ 71.28% (67/94) vs 4.17% ( 1/24 ) and 52.13% (49/94) vs 0% (0/24) ]. There were significant correlations between PI3K expression and the degrees of differentiation,invasive depth, clinical staging and the metastasis of lymph node ( all P 〈 0.01 ). The positive expression rate of MMP-7 had the relationship with metastasis of lymph node ( P 〈 0.05 ) , the degrees of differentiation ( P 〈 0.05 ) invasive depth( P 〈 0.01 ) , and clinical staging( P 〈 0.05 ) . There was a positive relationship between PI3K and MMP-7 expression in this study (r = 0. 232 ,P = 0.025 ). Conlusions PI3K and MMP-7 play an important roles in carcinogenesis and progression of esophageal carcinoma and can be used as valuable biomarkers to evaluate biological characteristics in esophageal squamous cell carcinoma.
出处
《中国综合临床》
2009年第10期1083-1085,共3页
Clinical Medicine of China
基金
河北省普通高等学校强势特色学科肿瘤学建设经费资助[冀教高(2005)52号]
河北卫生厅医学科研基金(2007118)