衣藻叶绿体表达系统的研究

The Research of Expression System in Chlamydomonas reinharditii Chloroplast

真核藻类作为一种新型的蛋白表达系统,因其培养方法简单,成本低廉并且能大规模繁殖,最近几年成为人们关注的焦点。作为一种模式生物,单细胞的真核生物莱茵衣藻已经成为人们研究的重点。外源蛋白不仅能在衣藻核中进行表达而且也能在叶绿体中表达,但衣藻叶绿体的表达系统较之核表达有巨大的优越性。在到目前为止,已经有许多的药用蛋白在莱茵衣藻的叶绿体中成功表达的报道,证明了莱茵衣藻叶绿体作为生物反应器的能力。将对衣藻叶绿体的表达做详细的描述。

In recently few years, people pay growing attention on the unicellular green alga Chlamydomonas reinhardtii, because it offers the potential to produce high yields of recombinant proteins more rapidly and cost lower than traditional cell culture. Being a model organism, Chlamydomonas reinhardtii has been studied intensively over the last decades and offers now a complete toolset for genetic manipulation. Recombinant proteins ean express in nuclear and chloroplast, but expression in the nuclear is superior to expression in the chloroplast . Recently, the successful expression of several proteins with pharmaceutical relevance has been reported from the chloroplastic genome of Chlamydomonas reinhardtii, proving capacity of Chlamydomonas reinhardtii chloroplast as a bioreactor.