绿色糖单孢菌-木素过氧化物酶的发酵工艺研究

Studies of Fermentation Conditions of Lignin Peroxidases from Saccharomonospora viridis

本研究以一株中度耐热耐碱放线菌——绿色糖单孢菌(Saccharomonospora viridis)为研究对象,用16 L发酵罐对该菌进行了木素过氧化物酶(lignin peroxidases,LiP)的诱导发酵,确定了最适的产酶工艺条件:接种量为10%,C/N为1∶3,搅拌速度为250 r/min,通气量为5 L/min,通过控制通气量和调整搅拌转速,使溶氧维持在35%以上,此条件下绿色糖单孢菌较摇瓶实验提前将近24 h达到产酶高峰,酶活最高可达0.41 U/ml;同时在发酵罐中测定该菌株的生长曲线和代谢曲线以确定其发酵代谢规律。

In a 16 L fermentor, production conditions of LiP from Saccharomonospora viridis were investigated. The optimal operation conditions were determined as follows, fermentor agitation rate 250 r/min, ventilation amount 5 L/min, the seeded volume 10%, corresponding to C： N ratio of 1 ： 3. The maximum enzyme activity could reach 0.41 U/ml at 96 h while dissolution oxygen （DO） level maintained higher than 35% by enhancing aeration and changing agitation rate. The growth curve and metabolism curve of this strain was also determined in the fermentor.