银杏叶提取物对肝纤维化大鼠肝细胞凋亡的影响

The Effects of GbE on the Hepatocellular Apoptosis of Hepatic Fibrosis Rats

目的:研究银杏叶提取物(GbE)对CCl4肝纤维化大鼠肝细胞凋亡的影响,探讨银杏叶提取物抗肝纤维化的作用机制。方法:采用CCl4建立雄性SD大鼠肝纤维化动物模型,同时以不同剂量的GbE灌胃6周进行干预;光镜观察肝组织的病理学变化,TUNEL法检测肝细胞的凋亡情况,免疫组化法检测凋亡相关蛋白Bcl-2、Bax的表达。结果:模型组大鼠肝组织存在不同程度的炎症和纤维化,肝细胞的凋亡指数和Bax表达较正常组明显增高(P<0.01)、Bcl-2表达则较正常组明显减少(P<0.01);不同剂量的GbE能减轻CCl4肝纤维化大鼠肝组织的炎症和纤维化程度(P<0.01、P<0.05),促进肝组织中Bcl-2表达的同时抑制Bax的表达(P<0.01、P<0.05),减少肝细胞的凋亡(P<0.01)。结论:银杏叶提取物的抗纤维化作用可能是通过调节Bcl-2和Bax的表达,减少肝细胞的凋亡来实现的。

Objective： To study the effects of GbE on the hepatocellular apoptosis and to explore its anti - fibrostie mechanism. Methods：Hepatic fibrosis of Male SD rats were induced by CC14 ,then all the rats had been treated with different dose of GbE for 6 weeks. After the experiment, the pathological changes of liver were observed with optical mlcroscope, Hepatocyte apoptosis was detected with TUNEL assay and apoptosis related proteins were detected with immunohistoehemistry assay. Results ：The rat liver of model group has different degrees of inflammation and fibrosis. The hepatocyte apoptosis index and the expression of Bax in the model group were significantly higher than in the normal group（P 〈0. 01 ）, while the expression of Bc1-2 was significantly lower（P 〈0. 01 ） ,different doses of GbE ean obviously improve the pathological changes（P 〈0.01 or P 〈0.05） ,increase the expression of Bcl - 2,inhibit the expression of Bax（P 〈0.01 or P 〈 0. 05 ） and decrease the hepatocyte apoptosis （ P 〈 0.01 ）. Conclusions ： The effect of GbE against fibrosis of rat liver may be related to regulating the expression of Bel -2 and Bax,and decreasing the hepatoeyte apoptosis of rats with liver fibrosis.