摘要
目的:探讨四妙勇安汤对实验性腹主动脉瘤(Abdominal Aortic Aneurysm,AAA)生长的影响及机制。方法:建立大鼠AAA模型,随机分成四妙勇安汤组和盐水对照组,造模后2周、4周,应用弹力纤维染色、CD68免疫组织化学染色观察动脉壁弹力纤维的损伤、巨噬细胞的浸润,用RT-PCR和Western蛋白质印迹方法观察单核细胞趋化蛋白-1(monocyte chemotacticprotein-1,MCP-1)及基质金属蛋白酶-2(matrix metalloproteinases-2,MMP-2)的基因表达。结果:与盐水对照组相比,造模后2周时,四妙勇安汤组大鼠腹主动脉的扩张、动脉壁弹力纤维的损伤、CD68阳性表达、MCP-1和MMP-2的基因表达程度均明显降低(P<0.01)。结论:四妙勇安汤能通过抑制动脉壁炎性细胞的浸润和相关基因的表达,对实验性腹主动脉瘤的生长产生明显的抑制作用。
Objective :To investigate the inhibition and its mechanism of Simiao Yong'an Decoction (SMYAD) on experimental abdominal aortic aneurysm. Methods : To establish rat AAA models, all rats were randomly divided into SMYAD group and blank control group. At 2 weeks and 4 weeks after operation, elastic fiber staining and CD68 immunohistochemical staining were used to observe the injury of elastic fibers and the infiltration of maerophages in the arterial wall, RT - PCR and Western blot were used to observe the gene expressions monocyte chemoattraetant protein - 1 ( MCP - 1 ) and matrix metalloproteinases - 2 ( MMP - 2), respectively. Results : Compared with the control group, the expansion of the abdominal aorta, the injury of elastic fibers in the arterial wall, CD68, MCP - 1 and MMP - 2 gene expression on SMYAD group were all significantly reduced at 2 weeks after operation ( P 〈 0.01 ). Conclusion : SMYAD has the obvious inhibiting effects on the growth of experimental abdominal aortic aneurysm by inhibiting the inflammatory cell infiltration and related gene expression in the arterial wall.
出处
《中华中医药学刊》
CAS
2009年第10期2161-2163,共3页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省教育厅资助项目(2004.F130)
辽宁省科技厅博士启动基金项目(20061030)
关键词
炎性细胞
四妙勇安汤
腹主动脉瘤
基因表达
inflammation cells
Simiao Yong'an Decoction
abdominal aortic aneurysm
gene expression