促性腺激素上调NF-κB的表达促进卵巢癌细胞的增殖

Gonadotropins enhances proliferation of ovarian cancer cells through up regulation of NF-κB

目的：探讨促性腺激素及核转录因子-κB（NF-κB）对卵巢癌细胞增殖的影响。方法：不同剂量的人重组促性腺激素与卵巢癌细胞株OVCAR-3、Caov-3共培养，应用四甲基偶氮唑蓝（MTT）实验观测其对卵巢癌细胞生长的影响，同时应用RT—PCR和ELISA方法检测卵巢癌细胞NF-κB水平，以分析其信号传导途径。结果：2种促性腺激素对卵巢癌细胞株均有促增殖作用，分别增加35％-202％（P〈0．05）；68％～192％（P〈0．01），并使细胞内NF-κB亚单位表达增加1．53～3．98倍（P〈0．05）；1．5-2．5倍（P〈0．01），与对照组相比差异具有显著性。在处理组卵巢癌细胞培养液中加入NF-κB抑制剂吡咯烷二硫代氨基甲酸盐（PDTC），上述2种卵巢癌细胞株的增殖均有所减慢。结论：促性腺激素对卵巢癌细胞株的促增殖过程可能由NF-κB介导，共同影响卵巢癌细胞的生长。

Objective： To investigate the effects and the primary mechanisms of gonadotropin and nuclear factor kappa B （NF-κB） on proliferation of human ovarian epithelial cancer cells. Methods： Two epithelial ovarian carcinoma cell lines （OVCAR-3, Caov-3） were co-cultured with follicle-stimulating hormone （FSH） or luteinizing hormone （LH） respectively. The degree of proliferation of the two cell lines was detected by MTT assay. The expression of mRNA and protein of NF-κB in cells were determined by RT- PCR and ELISA. Results： A dose-dependant effect was seen with gonadotropins in OVCAR-3 and Caov-3 （35%-202% ,P 〈0.05 ; 68%-192% ,P 〈0.01 respectively ）. The levels of expression of NF-κB mRNA and protein were also increased in these two kinds of cells （ 1.53±3.98 fold, P 〈0.05 ; 1.5-2.5 fold,P 〈0.01 respectively）by FSH and LH for a period of 96 h （P〈0.05 vs. control group）. Most significantly, for both cell lines, 100 μmol/L NF-κB inhibitor （PDTC） partially inhibited the hormones-stimulated cell proliferation. Conclusion： The results suggest NF-κB is possibly responsible for gonadotropins-stimulated proliferation of ovarian cancer cells.