肝细胞癌患者外周血单个核细胞IFN-γ和IL-10mRNA表达水平的检测

Expression of IFN-γ,IL-10mRNA in peripheral blood mononuclear cells from patients with Hepatocellular Carcinoma

目的:探讨外周血单个核细胞(PBMCs)γ干扰素(IFN-γ)和白细胞介素-10(IL-10)mRNA水平在肝细胞癌(HCC)发病中的作用。方法:分离20例HCC患者和20例健康体检者外周血PBMCs,提取总RNA,利用半定量RT-PCR方法逆转录并扩增IFN-γ和IL-10,通过凝胶图像扫描系统对PCR产物的电泳条带进行密度扫描。结果:HCC患者PBMCs中IL-10mRNA水平明显高于健康体检者(P<0.01),而IFN-γmRNA水平明显低于健康体检者(P<0.01)。结论:肝癌患者外周血PBMCs存在Th1/Th2失衡。

Objective： To analyze the expression levels of interferon gamma （IFN-γ） and interleukin 10 （IL-10） mRNA in peripheral blood mononuclear cells （PBMCs） in the pathogenesis of hepatocellular carcinoma （HCC）. Methods： PBMCs were isolated from 20 patients with HCC and the total RNA was extracted. The expression levels of IFN-γ and IL-10 mRNA in PBMCs were amplified by using semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）. The density of electrophoresis band in PCR products was scanned by gel scanning system. The results were compared with that from normal healthy volunteers. Results： RT-PCR analysis showed that the expression levels of IL-10 mRNA were significantly increased in patients with HCC compared with that in healthy individuals （P〈0.01）. Whereas IFN-γ mRNA levels were significantly decreased compared with that in heahhy individuals （P〈0.01）. Conclusion： Th1/ Th2 is imbalance in PBMCs with HCC.