摘要
目的探讨正弦磁场对提取的骨骼肌肌质网（SR）囊泡钙释放通道（RyR1）的影响。方法采用动态光谱法和同位素标记方法，分别检测了正弦磁场对SR囊泡Ca^2＋释放初速率、[3H]-Ryanodine平衡结合度、还原型辅酶（NADH）的氧化初速率和超氧（O^-·2）产率的影响。结果0．4mT、50Hz正弦磁场辐照SR囊泡30min，由1 mmol/L NADH与1 mmol／LNAD调控的Ca^2＋释放初速率Con组为（10．82周贞洁0．89）pmol·mg^-1 pro·s^-1、MF组为（14．69±1．21）pmol·mg^-1pro·s^-1；上调了35％，[^3H]-Ryan—odine平衡结合度上调15％，由（2．13±0．05）pmol／mg pro上升到（2．45±0．07）pmol／mg pro。另外，该磁场辐照SR囊泡30min，导致NADH的氧化速率上调22％，由（0．88±0．11）×10^-4 FI／s上升到（1．07±0．13）×10^-4 FI／s；O^-·2产率上调32％，由（0．99±0．09）×10^-5 Fl／s上升到（1．31±0．06）×10^-5 FI／s。结论0．4mT正弦磁场2在低氧化还原电势环境下对RyR1有显著的激活，且该磁场促进了NADH氧化酶的活力和O^-·2产率的增加。
Objective To investigate the effects of sinusoidal magnetic field on isolated sarcoplasmic reticulum （SR） calcium release channel （RyR1） function. Methods With the Ca^2± dynamic spectrum and isotope labeled methods, the Ca^2± release and [^3H]-Ryanodine binding, the initial rates of NADH oxidation and the production of superoxide of SR exposed to 50 Hz sinusoidal magnetic field （MF） were investigated respectively. Results 0.4 mT, 50 Hz sinusoidal MF exposure for 30 min increased SR Ca^2± release initial rate about 35% from （10.82±0.89）pmol·mg^-1 pro· s^-1 to （14.69-±1.21）pmol·mg^-1 pro· s^-1; and the [^3H]- Ryanodine binding by about 15% from （2.13±0.05）pmol ling pro to （2.45±0.07）pmol/mg pro, which regulated by 1 mmol/L NADH with 1 mmol/L NAD^＋ Meanwhile MF upregulated the rate of NADH oxidation by about 22% from （0.88±0.11 ） × 10^-4 FI/s to （ 1.07 ±0.13 ） × 10^-4 FI/s and upregulated the production of superoxide by about 32% from （0.99±0.09）×10^-5 FI/s to （1.31±0.06）×10^-5 FI/s. Conclusion 0.4 FI/s sinusoidal MF increases the activity of RyR1 within the low redox potential environment, and promotes NADH oxidase activity and superoxide production.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2009年第9期549-552,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
安徽省教育厅自然科学研究项目资助（20006kj272B）
