TLR2和TLR9在生殖器疱疹患者外周血单一核细胞的表达

Expressions of Toll-like receptor （TLR） 2 and TLR9 in peripheral blood mononuclear cells from patients with herpes zoster

目的探讨Toll样受体TLR2和TLR9在生殖器疱疹中的作用。方法流式细胞仪检测生殖器疱疹患者单一核细胞上TLR2和TLR9表达水平，同时采用ELISA法检测其血清IL-6、IL-10和TNF-α水平，并探讨其相关性。结果①生殖器疱疹患者PBMC上TLR2和TLR9表达水平明显高于正常成人（P〈0．05）。②生殖器疱疹患者血清IL-6和IL-10水平明显升高，TNF-α水平明显降低，与对照组比较，差异有统计学意义（P〈0．05）。③生殖器疱疹患者TLR2和TLR9表达水平与其血清IL-6，IL-10水平存在线性相关关系（P〈0．01）。结论TLR2和TLR9介导生殖器疱疹血清IL-6、IL-10的产生和释放。

Objective To study the expression level of TLR2 and TLR9 in peripheral blood mononuclear cells （PBMC） from patients with genital herpes （GH）. Methods Venous blood samples were collected from 20 patients with GH and 10 normal human controls. Flow cytometry was used to detect the expression levels of TLR2 and TLR9 in PBMC from these samples. Serum levels of IL-6, IL-10 and TNF-α were measured by ELISA. Results The percentage of PBMCs expressing TLR2 or TLR9 was significantly higher in patients than in normal human controls （18.88%±15.31% vs 7.49%±1.55%, 85.29%±3.15% vs 22.23%±2.38%, both P 〈 0.05）. Compared with the normal human controls, increased serum levels of IL-6 and IL-10 were observed in patients （91.25±3.22 pg/mL vs 6.65±1.27 pg/mL, 3.94±0.57 pg/mL vs 2.13±0.24 pg/mL, both P 〈 0.05）, together with a decrease in the serum level of TNF-oL （ 12.07 ±1.27 pg/mL vs 23.06±4.62 pg/mL）. The expression levels of TLR2 and TLR9 significantly correlated with serum levels of IL-6 and/L-10 in patients with GH （all P 〈 0.01 ）. Conclusion The production and release of serum IL-6 and IL-10 might be mediated by TLR2 and TLR9 in patients with genital herpes.