胃癌组织胸苷磷酸化酶和缺氧诱导因子-1α表达及其与预后相关性的研究

Expressions of TP and HIF-1α in gastric carcinoma tissues and their correlations with prognosis

目的:探讨胃癌组织胸苷磷酸化酶(TP)和缺氧诱导因子-1α(HIF-1α)的表达及与预后的相关性。方法:选取胃癌标本62例及20例正常胃黏膜组织,进行免疫组化检测TP、HIF-1α表达,并将结果与患者术后生存时间进行比较。结果:TP及HIF-1α在62例胃癌组织的阳性表达率分别为69.00%和66.00%。TP表达与肿瘤大小(χ2=8.988,P=0.030)、浸润深度(χ2=8.240,P=0.040)、淋巴结转移(χ2=13.964,P=0.000)、远处转移(P=0.047)和PTNM分期(χ2=6.066,P=0.014)相关。HIF-1α表达则只与肿瘤浸润深度(χ2=5.885,P=0.015)、淋巴结转移(χ2=4.353,P=0.037)和PTNM分期(P=0.029)相关。TP与HIF-1α的表达不相关,rs=0.205,P=0.110。TP、HIF-1α阳性者5年生存率明显低于阴性者,P<0.05。多因素分析表明,淋巴结转移、远处转移、HIF-1α的表达及PTNM分期是胃癌患者独立预后因子。结论:TP、HIF-1α在胃癌组织中高表达,但两者不相关。TP、HIF-1α过表达与胃癌的不良生物学行为有关,可能成为预测胃癌侵袭、发展及判断预后的重要指标。

OBJECTIVE： To explore the expressions of thymi dine phosphorylase （TP） and hypoxia inducible factor -1α（HIF-1α） in gastric carcinoma, and analyze their correlations with clinieopatholog ic features and prognosis. METHODS： The expressions of TP and HIF-1α in 62 cases of gastric carcinoma specimens and 20 normal gas tric tissues were examined by the immunohistochemical method. The postoperative survival rate was observed in these patients. RESULTS： The positive rates of the expressions of TP and HIF-1α protein were 69.00 % and 65.00 % in 62 patients with gastric carcinoma respective ly. TP expression was positively related to the tumor size （x^2 = 8. 988,P=0. 030）, depth of invasion（x^2= 8. 240,P= 0. 040）, lymph node metastasis （x^2 13. 964,P=0.000）, distant metastasis （P= 0.047）, and clinical stage （x^=6.066,P=0.014）. HIF-1α was only related to the depth of tumor invasion （x^2 = 5. 885, P= 0.015）, lymph node metastasis （x^2=4.353,P =0.037）, and clinical stage （P=0. 029）. There was no relationship between the expressions of TP and HIF-1α （rs =0. 205, P=0. 110）. Moreover, the 5 year survival rate was significantly better in the TP/ HIF-1α-negative group than that in the TP/HIF-1α/ positive group （P〈0.05）. Multivariate analysis showed that lymph node metastasis, distant metastasis, and the expressions of HIF-1α and PTMN stage were independent prog nostic factors in patients with gastric carcinoma. CONCLUSIONS： TP and HIF-1α are overexpressed in gastric carcinoma, but there is no correlation between TP expression and HIF la expression. The over expressions of TP and HIF-1α are related to the poor biological behav ior and prognosis of gastric carcinoma.