红细胞生成素对体外培养许旺细胞的作用

Effects of erythropoietin on Schwann cells in vitro

目的观察促红细胞生成素（erythropoietin，EPO）对体外培养许旺细胞分泌胶质细胞源性神经营养因子（GDNF）和细胞周期的影响，探讨EPO促进外周神经再生的可能机制。方法将原代培养、纯化的许旺细胞随机分成三组：A组（10％胎牛血清DMEM液）、B组（10％胎牛血清DMEM液加10U／mlEPO），C组（10％胎牛血清DMEM液加50U，／ml EPO），应用ELISA测定培养上清液中的GDNF水平，流式细胞术检测细胞周期分布。结果与A组相比，B组和C组培养上清液中的GDNF明显增高，许旺细胞处于S期百分比（S％）和（S＋G2M）％都有明显升高。结论EPO可增加体外培养许旺细胞分泌GDNF，并且能提高许旺细胞的增殖活性，这可能是其促进外周神经再生的重要机制之一。

Objective To observe the effects of EPO on GDNF secretion and cell cycle of Schwann cells（SCs） in vitro, and explore how EPO improve peripheral nerve regeneration. Methods Devided the purified Schwann cells of primary culture into three groups： group A with DMEM culture solution contained 10% fetal bovine serum and without EPO, group B with DMEM culture solution as above and 10 U/ml EPO, group C with DMEM culture solution as above and 50 U/ml EPO. The GDNF level of each group was detected in the culture by ELISA, and carried out the flow cytometry of Schwann cells on each group. Results GDNF in culture solution of group B, group C was more than that of group A, and the S-stage rate（S%） and （S ＋ G2M）% of Schwann cells in group B and group C more than that of group A. Conclusion EPO can increase the GDNF secretion and enhance proliferation activity of Schwann cells,which can explain how EPO improve peripheral nerve regeneration.