筋骨草总黄酮对慢性血清病肾炎大鼠自由基损伤的影响

Experimental Effects Study of Total Flavonoids of Ajuga on Free Radical Damage in Chronic Serum Sickness Glomerulonephritis Rats

目的:探讨筋骨草总黄酮对慢性血清病肾炎大鼠的治疗作用及其作用机制。方法:采用大鼠改良慢性血清病肾炎模型,于造模第5周末检测尿蛋白,将尿蛋白阳性者随机分为模型组、雷公藤多苷组、筋骨草总黄酮组,另设正常对照组。于造模第6周起开始给药,6周后,测定各组大鼠的24h尿蛋白定量、血生化和血清中SOD、MDA的含量,光镜下观察肾组织病理形态学变化。结果:与模型组相比,筋骨草总黄酮组可显著降低尿蛋白[(38.91±10.62)mg/24hvs(18.28±8.07)mg/24h,P<0.01],且可明显升高TP、SOD,降低BUN、Scr、TG、TC、MDA(P<0.05或P<0.01);形态学观察也显示筋骨草总黄酮组较模型组损害减轻[(0.196±0.035)vs(0.304±0.059),P<0.01]。而筋骨草总黄酮组与雷公藤多苷组相比,可明显提高SOD活性[(0.379±0.042)U/mlvs(0.327±0.063)U/ml,P<0.05],显著降低TC[(1.63±0.23)mmol/Lvs(1.95±0.37)mmol/L,P<0.05]、MDA含量[(4.773±0.675)U/mlvs(6.526±2.727)U/ml,P<0.05];且筋骨草总黄酮组ALT明显低于雷公藤多苷组[(57.60±8.09)U/Lvs(83.40±11.53)U/L,P<0.01]。结论:筋骨草总黄酮对慢性血清病肾炎大鼠有较好的治疗作用,而提高SOD活性、降低MDA、抑制脂质过氧化反应、减轻自由基损伤等可能是其治疗作用机制之一。

Objective：To investigate the effects of total flavonoids of Ajuga （TEA） in chronic serum sickness glomeru- lonephritis rats and its mechanism. Methods： Chronic serum sickness glomerulonephritis （CSS - GN） rat was induced by the improved chronic serum sickness model after five weeks, the positive urinary protein rats were randomly divided into model group, triptergium wilfordii polyglycosidium （TWP） group and TFA group, normal rats was considered as the control group. CSS - GN rats were treated with drugs since the sixth week; succeeding six weeks later, all rats were detected 24 h urinary protein,blood biochemistry, serum SOl）, MDA, and pathomorphological changes in renal tissue under microscopy were observated. Results： Compared with model group, TFA can significantly reduce the urinary protein [ （38.91 ± 10.62） mg/24 h vs （ 18.28 ±8.07） mg/24 h, P 〈 0.01 ], significantly increase TP and SOD, decrease BUN, Scr, TG, TC, and MDA （ P 〈 0.05 or P 〈 0.01 ）, morphological observation shows that TFA can mitigate the damage more than those in model group[ （0. 196 ± 0. 035）vs（0. 304 ± 0. 059）, P〈 0.01 ] ; Compared with TWP, TFA can significantly increase the SOl） activity [ （0. 379 ± 0. 042 ） U/mlvs（ 0. 327 ±0. 063 ） U/ml, P 〈 0.05 ], notably reduce Tch [ （ 1.63± 0.23）mmol/L vs （ 1.95 ± 0.37）mmol/L, P〈 0.05] and MDA [ （4. 773± 0. 675）U/ml vs （6. 526 ±2. 727）U/ml, P 〈 0.05 ] ; moreover, ALT can be significant decreased [ （57.60 ± 8.09） U/L vs （83.40 ±11.53 ） U/L, P 〈 0.01 ]. Conclusion： Total flavonoids of Ajuga have a therapeutic effect on CSS- GN rats;Reducing damage of free radical through SOD activity increasing, MDA lowering, and lipid peroxidation inhibition, may be one of the mechanisms of CSS- GN treatment.