失重降低大鼠腓肠肌介电性能

Reduction of Dielectric Properties of Rat Gastrocnemius Induced by Loss of Weight

利用Agilent4294A阻抗分析仪测量了离体大鼠腓肠肌的交流阻抗,通过频域介电谱、Cole-Cole图、损耗因子频谱、损耗角正切频谱和电导率虚部频谱的数据分析,观察模拟失重对大鼠离体腓肠肌细胞介电特性的影响。结果表明,模拟失重10周大鼠腓肠肌的低频介电常数εL降低,介电增量Δε减小,绝缘性降低;高频电导率κh降低,电导率增量Δκ降低,导电性降低;第一特征频率fC1减小,第二特征频率fC2增加;介电损失最大值Δε″max、损耗角正切最大值Δtgδmax和电导率虚部最大值Δκ″max均降低。提示失重导致的骨骼肌细胞介电性能下降。

In this experimental study, the AC impedance of isolated gastrocnemius was measured with an impendance analyzer of Agilent 4294A, and the effect of simulated weightlessness on the dielectric properties of the cells in isolated rat sural muscle was investigated by analyzing the dielectric numerical characters with the use of dielectric spectroscopy, the Cole-Cole plots, the spectrum of loss factor and loss tangent, as well as the spectrum of conductivity imaginary part. The results demonstrated that 10 weeks＇ simulated weightlessness caused some changes; for example, both permittivity at low frequency （εL） and permittivity increment（△ε） were reduced, and conductivity at high frequency（κb） was also reduced; at the same time, conductivity increment（△κ） was reduced, too. The first characteristic frequeney（fc1 ） decreased, while the second characteristic frequency（fc2） increased. All of the peak of loss factor, the peak of loss tangent, and the maximum of conductivity imaginary part were reduced. These data indicated that the reduction of dielectric properties of skeletal muscles was induced by weightlessness.