摘要
研究neuroplastin在培养神经元的表达及对大鼠原代培养小脑颗粒细胞分化与存活的影响。培养大鼠小脑颗粒细胞、中脑多巴胺能神经元、海马神经元、PC12-E2、新生鼠前脑组织,用RT-PCR检测neuroplastin65 mRNA和neuroplastin55 mRNA的表达;加入neuroplastin合成肽,观察小脑颗粒细胞神经突起生长及在低钾的神经毒性条件下神经元的存活。结果表明:在培养0、7d的海马神经元、中脑多巴胺能神经元、小脑颗粒细胞以及新生鼠前脑组织、PC12-E2细胞均表达np65 mRNA、np55 mRNA,且np55 mRNA的表达高于np65 mRNA;来自neuroplastin Ig1的合成肽是1ab-s,1cd-s,1dd,1ef,1fg;来自neuroplastinIg2的合成肽是2cd和2fg。1fg,1cd-s,2cd,2fg强烈刺激神经突起的生长,1dd,1ab-s中等程度刺激神经突起的生长,1ef,1bc则无效。1cd-s,1bc,1dd,1ef,2cd,2fg能促进低钾神经毒性环境下神经元的存活,1fg,1ab-s无效。以上结果提示,neuroplastin通过亲同性结合或亲异性结合后,诱导神经突起生长和促进神经元存活。
To investigate ncuroplastin mRNA expression and effects of neuroplastin on neurite outgrowth and neuronal survival in primary rat neurons, the neuroplastin 65 mRNA and neuroplastin 55 mRNA were detected in cerebellar granule neurons （ CGN）, dopaminergic neurons, hippocampal neurons in primary） culture and forebrain, PC12-E2 hy RT-PCR. The neurite outgrowth and survival in primary CGN were investigated when the ncuroplastin-derived peptide was present in the medium. Results showed that CGN, dopaminergic neurons and h ippocampal neurons in fi＇esh and 7 days in culture and forebrain, PC12-E2 all expressed np65 mRNA and np55 mRNA. The level of np55 mRNA was higher than that of np65 mRNA in these samples, 1ab-s, 1cd-s, 1dd, 1ef and 1fg are neuruplastin Ig1-derived peptides, 2cd and 2fg are neuroplastin Ig2-derived peptides, 1fg, 1cd-s, 2cd, 2fg strongly induced the neurite outgrowth of primary CGN, 1 dd and labs mediately induced ncurite outgrowth, but 1 ef and 1 bc did not induce neurite outgrowth. 1 cd-s, 1bc, 1 dd, 1ef, 2cd and 2fg promoted the CGN survival upon induction of cell death in concentration of potassium in the cultures, but 1fg and Iabs did not promote the CGN survival in the same condition. Our data indicate that neuroplastin, by homophilic interaction and heterophilic binding, can induce neurite outgrowth and protect neuronal survival.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2009年第5期546-552,共7页
Chinese Journal of Neuroanatomy
