脑脉泰对慢性脑缺血致血管性痴呆大鼠脑血流量及海马神经元凋亡的影响

Effects of Naomaitai Capsule on cerebral blood flow and apoptosis of hippocampus neuron in rats with vascular dementia induced by chronic forebrain ischemia

目的研究慢性脑缺血致血管性痴呆大鼠海马区局部脑血流量(rCBF)改变及细胞凋亡情况,探讨脑脉泰治疗血管性痴呆的疗效及可能机制。方法采用双侧颈总动脉永久结扎法(2VO)制备慢性脑缺血致血管性痴呆的动物模型,随机分为假手术对照组(12只)、模型对照组(12只)、给药组(脑脉泰)(12只)。手术后4周,HE染色观察神经元形态学改变,免疫组化染色检测海马区Caspase-3、bax蛋白表达变化。应用激光多普勒血流仪检测各组大鼠海马局部脑血流量。结果模型对照组大鼠海马Caspase-3、Bax阳性细胞灰度平均值低于假手术对照组、给药组,差异均具有显著性(P<0.05);模型对照组海马rCBF低于假手术对照组、给药组,差异均具有显著性(P<0.05)。结论VaD模型大鼠海马rCBF下降,海马神经细胞凋亡增加;脑脉泰能增加实验大鼠海马局部脑血流量,抑制海马神经细胞凋亡。

Objective To study the changes of region cerebral blood flow （rCBF） and apoptosis of hippocampus neuron in rats with vascular dementia （VaD） induced by chronic forebrain ischemia and the effects of Naomaitai capsule on them, and explore the possible mechanisms. Methods The rat model of VaD was prepared by permanent occlusion of bilateral common carotid arteries （2VO）. Wistar rats were randomly divided into sham-operated control, model control and Naomaitai capsule-treated groups. After 4 weeks of the operation, the morphologic changes of the hippocampus neuron was observed with HE staining. The expression of Caspase-3 protein and Bax protein were detected by immunohistochemistry. The rCBF of the hippocampus was assessed by laser Doppler. Results Compared with the sham-operoted control and the Naomaitai capsule-treated groups, the mean gray values of the Caspase-3 protein and Bax protein in the model control group decreased （ P 〈 0. 05 ）, and the rCBF in the model control group was also lower than that in the other two groups （P 〈 0.05）. Conclusions The rCBF of hippocampus decreased and apoptosis increased in VaD rats. Naomaitai capsule can increase the rCBF of hippocampus, and inhabit the apoptosis of hippocampus neurons.