摘要
应用末端脱氧核糖核酸转移酶(TdT)介导的dUTP缺口末端标记技术(TUNEL)、碘化丙锭染色后流式细胞仪分析两种方法观察35例骨髓增生异常综合征(MDS)、10例正常人的凋亡率.MDS中难治性贫血(RA)、环形铁粒幼细胞性难治性贫血(RAS)、难治性贫血伴原始细胞增多(RAEB)凋亡率显著高于正常人(P<0.01),难治性贫血伴原始细胞增多-转变型(RAEB-T)凋亡率无明显增高,与正常对照组无明显差异(P>0.05).说明MDS中RA、RAS、RAEB存在凋亡过度,提示凋亡过度为MDS无效造血机制之一,病情演变至RAEB-T时转为凋亡障碍.抗凋亡疗法可试用于早期MDS.
The apoptosis in bone marrow mononuclear cells of 35 patients of myelodysplastic syndromes （MDS） and 10 normal controls are evaluated separately by using TDT- mediated dUTP nick end labeling method and propidium iodine staining flow cytometric analysis. The apoptosis rate drastically rises in RA,RAS,RAEB of MDS（ P 〈 0.01 ）, but RAEB - T of MDS exhibits a normal apoptosis（P 〉 0.05）. This study shows that RA, R.AS, RAEB of MDS has an excessive apoptosis which is one of the factors leading to ineffective hematopoiesis in MDS. When progressing to RAEB- T, the apoptosis is hindered. Therapy of anti - apoptosis can be applied to early MDS patients.
出处
《绍兴文理学院学报》
2009年第9期33-36,共4页
Journal of Shaoxing University
基金
绍兴文理学院科研项目(07LG1034)
关键词
骨髓增生异常综合征
凋亡
骨髓
myelodysplastic syndromes
apoptosis
bone marrow
