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转染Smac增强非小细胞肺癌细胞株A549对顺铂的化疗敏感性 被引量：4

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摘要 目的探讨转染Smac对非小细胞肺癌细胞株A549化疗敏感性的影响。方法利用脂质体将PcDNA3.1/Smac重组体转染到非小细胞肺癌细胞株A549,并联合顺铂作用于A549,western blot检测转染后细胞内Smac蛋白表达。Annexin V/PI双染经流式细胞仪(FCM)检测细胞凋亡率。结果(1)western blot检测结果:可见转染PcDNA3.1/Smac重组体后细胞内Smac蛋白表达明显高于未转染组(P<0.05)。(2)单独转染PcDNA3.1/Smac仅能诱导较少细胞凋亡,但与空载体PcDNA3.1转染组及未处理组比较差异有显著性(P<0.05)。(3)转染PcDNA3.1/Smac并顺铂(5μg/mL)联合作用,细胞凋亡率明显较单独使用顺铂组升高(P<0.05)。结论转染Smac能增强非小细胞肺癌细胞株A549对顺铂的化疗敏感性。
作者 成先东 蔡绍曦 赵海金 李文军 佟万成
机构地区 南方医科大学南方医院呼吸内科 赣南医学院第一附属医院 南方医科大学公共卫生与热带医学学院
出处 《广东医学》 CAS CSCD 北大核心 2009年第10期1458-1460,共3页 Guangdong Medical Journal
基金 广东省名医工程研究项目(编号:粤卫[2004]199-7)
关键词 SMAC 非小细胞肺癌 转染 凋亡 顺铂
分类号 R734.202 [医药卫生—肿瘤] R730.53 [医药卫生—肿瘤]
  • 相关文献

参考文献8

  • 1IGNEY F H, KRAMMER P H. Death and anti - death: tumour resistance to apoptosis[J]. Nat Rev Cancer, 2002, 2(4) : 277 - 288.
  • 2SRINIVASA M S, DATTA P, XUN J U, et al. Molecular determinants of the caspase - promoting activity of Smac/DIABLO and its role in the death receptor pathway [ J ]. Biol Chem, 2000, 275 : 36 152.
  • 3DU C, FANG M, LI Y, eta]. Smac, a mitoehondrial protein that promotes cytochrome c - dependent caspase activation by eliminating IAP inhibition[J]. Cell, 2000, 102(1 ) : 33 -42.
  • 4GUO F, NIMMANAPALLI R, PARANAWITHANA S, et al.Ectopic overexpression of second mitoehondria derived activator of caspases ( Smac /DIABLO) or cotreatment with N - terminus of Smac/DIABLO peptide potentiates epothilone B derivative (BMS 247550) and Apo -2L/TRAIL2 - induced apoptosis[J]. Blood, 2002, 99(9) : 3 419 -3 426.
  • 5FULDA S, WICK W, WELLER M, et al. Smac agonists sensitize forApo2L/TRAIL or anticancer drug - induced apoptosis and induce regression of malignant glioma in vivo [J]. Nat Med, 2002,8(8): 808 -815.
  • 6郑丽端,童强松,陶凯雄,汪良,张波.Smac基因过表达对胃癌细胞株MKN-45化疗敏感性的影响[J].癌症,2004,23(4):361-366. 被引量：11
  • 7SEKIMURA A, KONISHI A, MIZUNO K, et al. Expression of Smae/DIABLO is a novel prognostic marker in lung cancer [ J ].Oncol Rep, 2004, 11 (4) : 797 - 802.
  • 8成先东,蔡绍曦,赵海金,李文军,佟万成.Smac在顺铂促非小细胞肺癌细胞株凋亡中的作用[J].南方医科大学学报,2008,28(3):389-391. 被引量：6

二级参考文献13

  • 1施春雷,韩宝惠.ASCO不可手术切除的非小细胞肺癌治疗指南——2003新版推荐指南[J].循证医学,2004,4(2):115-124. 被引量：114
  • 2赵进明,朱志华,吴秋良,侯景辉,黄晓平,张蓬原,戎铁华.Smac在Ⅰ～Ⅱ期非小细胞肺癌组织中的表达及其临床意义[J].癌症,2006,25(5):631-634. 被引量：8
  • 3Vaux DL,Silke J. Mammalian mitochondrial IAP binding proteins [J]. Biochem Biophys Res Commun,2003,304(3):499- 504.
  • 4Verhagen AM,Vaux DL. Cell death regulation by the mammalian IAP antagonist Diablo/Smac [J]. Apoptosis, 2002,7(2):163- 166.
  • 5Song Z,Yao X,Wu M. Direct interaction between survivin and Smac/DIABLO is essential for the anti-apoptotic activity of survivin during taxol-induced apoptosis [J]. J Biol Chem,2003,278(25):23130- 23140.
  • 6McNeish IA,Bell S,McKay T,et al. Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway [J]. Exp Cell Res,2003,286(2):186- 198.
  • 7Fulda S,Wick W,Weller M,et al. Smac agonists sensitize for Apo2L/TRAIL-or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo [J]. Nat Med,2002,8(8):808- 815.
  • 8Guo F,Nimmanapalli R,Paranawithana S,et al. Ectopic overexpression of second mitochondria-derived activator of caspases (Smac) or cotreatment with N-terminus of Smac peptide potentiates epothilone B derivative-(BMS 247550) and Apo-2L/TRAIL-induced apoptosis [J]. Blood,2002,99(9):3419- 3426.
  • 9Du C,Fang M,Li Y,et al. Smac,a mitochondrial protein that promotes cytochrome c-dependent caspase activation by elimination IAP inhibition [J]. Cell,2000,102(1):33- 42.
  • 10Jia L,Patwari Y,Kelsey SM,et al. Role of Smac in human leukaemic cell apoptosis and proliferation [J]. Oncogene,2003,22(11):1589- 1599.

共引文献15

同被引文献37

  • 1陈晓,杨春鹿,赵君,李大朋,张林.非小细胞肺癌组织Livin和Smac蛋白表达及其临床意义的研究[J].中华肿瘤防治杂志,2008,15(12):905-909. 被引量：24
  • 2赵进明,朱志华,吴秋良,侯景辉,黄晓平,张蓬原,戎铁华.Smac在Ⅰ～Ⅱ期非小细胞肺癌组织中的表达及其临床意义[J].癌症,2006,25(5):631-634. 被引量：8
  • 3Du C,Fang M,Li Y,et al.Smac,a mitochondrial protein that pro-motes cytochrome-dependent caspase activation by elimination IAP in-hibition[J].Cell,2000,102(1):33-42.
  • 4Jia L,Patwari Y,Kelsey SM,et al.Role of Smac in human leukaemiccell apoptosis and proliferation[J].Oncogene,2003,22(11):1589-1599.
  • 5Verhagen AM,Ekert PG,Pakusch M,et al.Identification of DIAB-LO,a mammalian protein that promotes apoptosis by binding to andantagonizing IAP proteins[J].Cell,2000,102(1):43-53.
  • 6Ogura A,Watanabe Y,Iizuka D,et al.Radiation-induced apoptosis oftumor cells is facilitated by inhibition of the interaction between Survivinand Smac/DIABLO[J].Cancer Lett,2008,259(1):71-81.
  • 7Pluta A,Wrzesien-Kus A,Cebula-Obrzut B,et al.Influence of highexpression of Smac/DIABLO protein on the clinical outcome in acutemyeloid leukemia patients[J].Leuk Res,2010,34(10):1308-1313.
  • 8Jemal A.Siegel R,Ward E. et al. Cancer statics,2008[J].CACancer J Clin,2008,58(2) :71-96.
  • 9Petersen SL. Wang L,Yalcin-Chin A.et al. Autocrine TNF alphasignaling renders human cancer cells susceptible to Smac-mimet-ic-induced apoptosis[J],Cancer Cell,2007,12(5) :445-456.
  • 10Varfolomeev E,Blankenship W,Wayson SM,et al,IAP antago-nists induce antoubiquitination of c-IAPs,NF-kappa Bactivation,and TNF alpha-dependent apoptosis[J].Cell,2007,131(4) :669-681.

引证文献4

二级引证文献25

广东医学
2009年 第10期

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