多头绦虫抗原特性的研究──应用IHA观察绵羊免疫及感染后的抗体消长规律

STUDIES ON THE ANTIGENS OF MUTICEPS CESTODE ─APPLICATION OF IHA FOR INVESTIGATION OF ANTIBODY RESPONSE IN SHEEP AFTER IMMUNIZATION AND INFECTION

为了观察绵羊用多头蚴抗原免疫及感染后的抗体消长规律，为羊脑多头蚴病的免疫预防和免疫诊断提供依据，本试验应用多头蚴原头节可溶性抗原、囊壁可溶性抗原、囊液粗抗原致敏绵羊红细胞对绵羊免疫3次及虫卵攻击感染后的血清抗体进行间接血凝试验（IHA）检测。结果表明，原头节抗原免疫组、囊壁抗原免疫组、囊液抗原免疫组及原头节ES抗原免疫组在首次免疫后1周，抗体滴度迅速升高，第3次免疫后1周达到峰值，虫卵感染后开始下降，到感染后30周接近正常水平。多头蚴3种抗原对同种抗原免疫组血清检测敏感性、特异性优于其它抗原，原头节免疫组、囊壁免疫组、囊液免疫组抗体水平明显高于原头节ES抗原免疫组。

In order to investigate antibody response in sheep after immunized by CoenurusCerebralis antigens and infected by multiceps multiceps eggs, the protoscolex soluble antigen,the cyst wall soluble antigen and the cyst liquid antigen were used in inderect haemagglutination assay (IHA) to detect serum of sheep after immunization and infection.The results showedthat the serum antibody of immunized groan rose rapidly after the first immune injection andreached the peak after the third injection.After infected with eggs the antibody began to decrease untill the 30 th week.The sensitivity and specificity of Coenurus cerebralis antigens werehigher than others in detecting the serum of sheep immunized with the same antigens.The antibody level of sheep immunized with protoscolex, cyst wall and cyst liquid antigens were higher than that of the sheep immunized with protoscolex ES antigen.