摘要
为了探明伊氏锥虫在动物体内抗原变异,以伊氏锥虫新疆株(XJCA)克隆感染兔,每3天采兔血1次,共采血5次,并用环磷酰胺处理的小白鼠对各分离期锥虫予以克隆,获得5个锥虫克隆群体,经ABC-酶标试验和间接免疫荧光试验,对各克隆锥虫抗原进行鉴定,结果:获得5个表面抗原性互不相同的克隆群体,说明伊氏锥虫新疆株第1次抗原变异发生在4~6日内,以后每3天变异1次,本研究为进一步探明伊氏锥虫抗原变异规律打下了基础。
To investigate antigenic variation of T. evansi infection in animal T. evansiXJCA strain was cloned and then infected rabbits, five periods rabbit blood was taken at intervals of 3 days, we got five cloned trypanosomes by CY mice cloning trypanosoma from bloodof infected rabbit. Five cloned trypanosoma was distinguished by ABC-EIA and immunofluorescence test the results showed that five cloned trypanosoma different in surface antigenic weregotten, the exertment indicated T. evansi took antigenic variation at the first 4-6 days andthen at intervals of 3 days, the study is the base for futher investigate the rule of T. evanisantigen vatiation.
出处
《中国兽医寄生虫病》
1998年第4期8-10,共3页
Chinese Journal of Veterinary Parasitology
关键词
伊氏锥虫
克隆
抗原变异
ABC-酶标
动物体
Trypanosoma evanis
Clone
Antigenic vatiation
ABC-EIA
Immunoluorescence