正己烷中脂肪酶催化合成三乙醇胺-硬脂酸酯

Synthesis of triethanolamine-stearic acid ester catalysed by lipase in n-hexane

Triethanolamine-stearic acid ester was synthesized in n-hexane by stearic acid and triethanolamine as substrates,acetone powder of lipase remembered pH value.The optimum conditions were as follow:the molar ratio of stearic acid to triethanolamine saturated with water was 3.5∶1,the weight ratio of purified lipase and stearic acid was 100∶1.For absorbing water produced,4A sizing screens were added after 8h,its quality was 1/4 of stearic acid.Yield of esterification was arrived to 71.42% at 60hs.Replacing 4A sizing screen with N2 which is at 0.05Mpa and 4000mL/h,the esterification yield arrived to 74.25% at 60h.Lipase had catalysing activity in lower polar solvent,and the esterification equilibrium arrived while the system water was exceed 36.2%.

Triethanolamine-stearic acid ester was synthesized in n-hexane by stearic acid and triethanolamine as substrates,acetone powder of lipase remembered pH value. The optimum conditions were as follow： the molar ratio of stearic acid to triethanolamine saturated with water was 3.5：1 ,the weight ratio of purified lipase and stearic acid was 100：1. For absorbing water produced ,4A sizing screens were added after 8h, its quality was 1/4 of stearic acid. Yield of esterification was arrived to 71.42% at 60hs. Replacing 4A sizing screen with N2 which is at 0.05Mpa and 4000mL/h,the esterification yield arrived to 74.25% at 60h. Lipase had catalysing activity in lower polar soh,ent, and the esterification equilibrium arrived while the system water was exceed 36.2%.