基于氧化锌-壳聚糖-纳米金复合杂化膜修饰的过氧化氢生物传感器的研究

A Hyrogen peroxide biosensor based on immobilizing Hemoglobin on gold nanoparticles and chitosan-Zinc oxide nanoparticles composite films

将氧化锌（ZnO）-壳聚糖（CHIT）-纳米金（nano—Au）形成复合杂化膜包埋血红蛋白（Hb），制备了无电子媒介体的第三代电流型生物传感器．该传感器对过氧化氢（H2O2）的还原显示出较好的电催化活性，固定在电极上的Hb在0．1mo／／L磷酸盐缓冲溶液（PBS）中对过氧化氢响应灵敏度高，检测范围宽（3．9×10^-6～1．907×10^-72mol／L），检测下限低（1．53×10^-6mol／L（信噪比S／N=3）），并且表现出良好的稳定性和高选择性．

The third generation amperometric hyrogen peroxide biosensor based on Chitosan - Zinc oxide nanoparticles composite films and nano - Au and Hemoglobin has been constructed. The electrode showed a high thermal stability, offering an excellent electrochemical response for determination of H2 02 concentration with high sensitivity and selectivity. The linear range for H2O2 determinationwas from 3.9 ×10^--6to 1. 907×10^-2mol/L with a detection limit of 1.53 ×10^-6mol/L of （S/N =3）.