碳源和氮源对大豆愈伤组织生长和染料木素积累的影响

The Influence of Carbon Source and Nitrogen Source to the Growth of Soy Callus and the Accumulation of Genistein

研究了不同碳源和氮源对大豆愈伤组织生长及染料木素含量积累的影响。结果表明：在6种所试验的碳源中，以蔗糖为单一碳源时总体效果最好，45g几左右的浓度对愈伤组织和染料木素的积累较为有利。培养基中{NO3]一比例较大时有利于愈伤的生长，[NH4}＋比例较大时有利于染料木素的积累，[NH4]＋／[NO3】-为10／50时，染料木素产量最高；总氮浓度为90mmol／L对愈伤生物量和染料木素的积累最为有利。．

This thesis makes a research on the influence of different carbon source and ni- trogen source to the ~owth of soy callus and the accumulation of genistein. The results show that, among the six carbon sources under experiment, the overall effects come to the best when su- crose, with the concentration of 45g/L, being the only carbon source and it＇s good for the callus and the accumulation of genistein. The bigger scale of [NO3]- in culture medium is good for the growth of callus and the bigger scale of [NH4]＋is good for the accumulation of genistein. When [NH4]＋/ [NO3]- is 10/50, the production of genistein ranks the highest; when the concentration of overall nitrogen is 90mmol/L, it is best for the callus biomass and the acculution of genistein.