人树突状细胞融合肝癌细胞体外诱导特异性抗肿瘤免疫

Specific cycotoxic effects against hepatocellar carcinimar by fusing human dendritic cells with heptic cancer cells

目的探讨人树突状细胞（DC）融合肝癌细胞（HCC）体外诱导T淋巴细胞产生特异性抗肝癌免疫的作用。方法应用人重组粒细胞／巨噬细胞集落刺激因子（rhGM—CSF）和重组人白细胞介素4（rhIL-4）对人外周血单个核细胞进行体外诱导产生树突状细胞，流式细胞仪检测DC表面标志物表达水平，聚乙二醇融合DC与肝癌细胞HerG2，MTT法测定融合细胞（HerG2／DC）刺激T淋巴细胞增生、分化能力，细胞毒性实验检测HerG2／DC诱导的细胞毒T淋巴细胞（CTL）对HerG2的特异性杀伤作用。结果融合细胞HerG2／DC刺激T淋巴细胞增值能力明显提高，HerG2／DC活化的CTL对HerG2具有明显的特异性杀伤作用。结论人树突状细胞融合肝癌细胞可有效诱导T淋巴细胞产生特异性的抗肝癌肿瘤免疫。

Objective To investigate the effect of fusions of hepatic cancer cell（HCC） with human dendritic cell （DC） induce autologous T lymphocytes on eliciting specific immunity against HCC. Methods DC isolated from human peripheral blood were cultured and proliferated for one week by using recombinant human granuiocyte/ macrophage-colony stimulating factor （rhGM-CSF） and interleukin-4 （rhIL-4）. Fusions of DC with HepG2 cells （HepG2/DC） were achieved by polythyleneglycol （PEG）. The ability of HepG2/DC to stimulate proliferation and differentiation of autologous T lymphocytes was assessed by MTT method and the specific killing efficacy of HepG2/ DC-induced cytotoxic T lymphocytes （CTL） to HepG2 was evaluated. Results After one week culture, DC present a high level expression of CD1 a, HLA-DR, CD54, CDS0, CD86. Fusions had remarkably greater ability to stimulate proliferation of autologous T lymphocytes in compare with HepG2, HepG2 ＋ DC and DC. The HepG2/DC activated CTL showed a potential specific killing efficacy to HepG2. Conclusion Fusions of HCC cells with DC can ef- fectively stimulate autologous T lymphocytes to elicit specific antitumor immunity against HCC.