丹参酮化合物对HeLa细胞抑制作用与构效关系探讨

Inhibition and Structure-Activity Relation of Tanshinones on Growth of HeLa Cell Line

目的研究丹参酮化合物对HeLa细胞增殖的抑制作用,阐明其结构与细胞毒活性的关联性。方法MTT比色法检测不同浓度的丹参酮化合物,分别在24,48,72 h对HeLa细胞增殖的抑制作用。结果不同浓度(0.5～16.0μg.mL-1)丹参酮ⅡA、丹参酮I、二氢丹参酮、隐丹参酮对HeLa细胞毒性均有明显剂量和时间依赖性,其作用24 h的IC50值分别为:24.1,20.1,8.4,17.8μg.mL-1;作用48 h的IC50值分别为:8.0,11.1,2.2,8.2μg.mL-1;作用72 h的IC50值分别为:4.6,3.5,1.5,8.1μg.mL-1。结论丹参酮化合物对HeLa细胞均具有增殖抑制作用,其中二氢丹参酮对宫颈癌细胞系HeLa的细胞毒性最强。A环为芳香环与D环呋喃环的结构差别使其对HeLa细胞增殖的抑制作用有明显的差异。

Objective To investigate the effects of tanshinones on growth of HeLa cell line and their structure-activity relationship. Methods MTT was used to measure the proliferation of HeLa cells cultured with tailshinones at different concentrations at 24 h,48 h and 72 h. Results The different concentrations （0.5-16.0 μg·mL^-1 ） of Tan Ⅱ A, Tan Ⅰ, dihydrotanshinone, eryptotanshinone had cytotoxieities on HeLa cells in a dose-dependent and time-dependent manners. , with IC50 of which were 24.1,20.1,8.4,17.8μg·mL^-1at 24 h; 8.0, 11.1,2.2,8.2 μg·mL^-1 at 48 h; 4.6,3.5,1.5,8. 1 μg·mL^-1 at 72 h, respectively. Conclusion Tanshinones have proliferating inhibition effect on HeLa cell line, the dihydrotanshinone among which is the most active one. The structure disparity between aromatic ring A and furan ring D may produce the difference on growth inhibition in HeLa cells.